首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠原癌基因Wnt-1蛋白(WNT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT1含量。WNT1 是一个重要靶点。它属于 Wnt 信号通路相关蛋白，在胚胎发育、细胞增殖分化等生理过程中发挥关键作用。研究发现其异常激活或表达与肿瘤等疾病发生发展相关，目前围绕其信号传导机制及阻断策略的研究持续推进。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研领域，如肿瘤发生机制研究、干细胞分化调控分析、Wnt信号通路相关药物开发等方向，支持用户开展细胞实验、动物模型或疾病样本的定量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Wnt1 ELISA kit; Int-1 ELISA kit; Wnt-1Proto-oncogene Wnt-1 ELISA kit; Proto-oncogene Int-1 ELISA kit
缩写：WNT1
Uniprot No.：P04426
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 106 | 100-110 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.7492.6272.6882.5436002.2342.3062.2702.1253001.4931.5341.5141.3691500.7290.7550.7420.597750.4010.4270.4140.26937.50.2770.2590.2680.12318.750.2360.2470.2420.09700.1470.1420.145 | ||||||
货期：3-5 working days
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