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产品描述：人WNT1诱导信号通路蛋白2(WISP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WISP2含量。WISP2是一个靶点。它在多种生理病理过程中发挥作用。研究机制上，其可能参与细胞增殖、分化、凋亡等调控。后续研究有望深入解析其信号通路，探究其在疾病发生发展中的具体机制，为相关疾病防治提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探索WNT信号通路调控机制、代谢相关疾病模型研究及肿瘤微环境中WISP2的功能分析，可为细胞实验、动物模型或生物样本的蛋白表达水平检测提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCN family member 5 ELISA Kit; CCN5 ELISA Kit; Connective tissue growth factor like protein ELISA Kit; Connective tissue growth factor related protein 58 ELISA Kit; Connective tissue growth factor-like protein ELISA Kit; Connective tissue growth factor-related protein 58 ELISA Kit; CT58 ELISA Kit; CTGF L ELISA Kit; CTGF-L ELISA Kit; WISP 2 ELISA Kit; WISP-2 ELISA Kit; Wisp2 ELISA Kit; WISP2_HUMAN ELISA Kit; Wnt 1 signaling pathway protein 2 ELISA Kit; WNT1 inducible signaling pathway protein 2 ELISA Kit; WNT1-inducible-signaling pathway protein 2 ELISA Kit
缩写：WISP2
Uniprot No.：O76076
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WISP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:10 | Average % | 103 | ||||
| Range % | 98-110 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human WISP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-102 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5912.7612.6762.50952.1242.1772.1511.9842.51.4511.4641.4581.2911.250.8320.7990.8160.6490.6250.4620.4430.4530.2860.3120.3110.3110.3110.1440.1560.2100.2240.2170.05000.1650.1680.167 | ||||||
货期：3-5 working days
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