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产品描述：人原癌基因Wnt-1蛋白(WNT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT1含量。WNT1是重要靶点。它是Wnt信号通路成员，在胚胎发育、细胞增殖分化等生理过程中起关键作用。异常激活或抑制会引发肿瘤、神经退行性疾病等。当前研究围绕其在疾病发生机制及阻断或激活该靶点以开发治疗药物。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于科研领域对WNT1蛋白表达水平的动态监测，例如肿瘤发生机制研究、Wnt信号通路功能解析、疾病动物模型评估或药物干预效果分析等，为探索WNT1在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMND16 ELISA Kit; INT1 ELISA Kit; OI15 ELISA Kit; oncogene Int1 ELISA Kit; Proto oncogene protein Wnt 1 ELISA Kit; Proto-oncogene Int-1 homolog ELISA Kit; Proto-oncogene Wnt-1 ELISA Kit; Wingless type MMTV integration site family member 1 ELISA Kit; wingless-type MMTV integration site family; member 1 (oncogene INT1) ELISA Kit; Wnt 1 ELISA Kit; wnt1 ELISA Kit; WNT1_HUMAN ELISA Kit
缩写：WNT1
Uniprot No.：P04628
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of human WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-94 | ||||
| EDTA plasma (n=4) | 99 | 96-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4992.6592.5792.415102.1452.0012.0731.90951.4021.5221.4621.2982.50.9440.9500.9470.7831.250.6660.6450.6560.4920.6250.4220.4540.4380.2740.3120.2890.2950.2920.12800.1580.1690.164 | ||||||
货期：3-5 working days
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