| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MTHFR; Methylenetetrahydrofolate reductase |
| Entrez GeneID | 4524 |
| WB Predicted band size | Calculated MW: 75 kDa; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human MTHFR |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MTHFR抗体的示例参考文献(注：以下内容为虚构，用于演示格式，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Autoantibodies Against MTHFR in Systemic Lupus Erythematosus: Correlation with Disease Activity*
**作者**: Lee J, et al.
**摘要**: 本研究检测了系统性红斑狼疮(SLE)患者血清中的抗MTHFR自身抗体，发现其阳性率显著高于健康对照组，且抗体水平与疾病活动指数(SLEDAI)呈正相关，提示MTHFR抗体可能参与SLE的病理机制。
2. **文献名称**: *Development of a Monoclonal Antibody for Specific Detection of MTHFR C677T Mutation Variants*
**作者**: Smith A, et al.
**摘要**: 研究团队开发了一种针对MTHFR C677T突变蛋白的单克隆抗体，该抗体能通过免疫组化区分突变型与野生型MTHFR，为临床样本的基因型-表型关联分析提供了新工具。
3. **文献名称**: *Anti-MTHFR Antibodies and Recurrent Pregnancy Loss: A Case-Control Study*
**作者**: Zhang Y, et al.
**摘要**: 本文探讨了复发性流产(RPL)女性中抗MTHFR抗体的流行情况，发现抗体阳性患者的血浆同型半胱氨酸水平升高，提示抗体可能通过抑制MTHFR酶活性导致叶酸代谢异常，从而增加流产风险。
4. **文献名称**: *MTHFR Autoantibodies in Neurological Disorders: Insights from an ELISA-Based Screening*
**作者**: Garcia R, et al.
**摘要**: 研究采用ELISA法检测多种神经系统疾病(如多发性硬化、神经病变)患者的抗MTHFR抗体，发现部分患者抗体滴度升高，且与神经炎症标志物相关，为探索治疗靶点提供了依据。
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**注意**：以上文献为示例性虚构内容，实际研究中关于MTHFR抗体的直接文献较少，建议结合关键词“MTHFR autoantibody”或“anti-MTHFR antibody”在PubMed、Google Scholar等平台检索最新成果。
The methylenetetrahydrofolate reductase (MTHFR) enzyme plays a critical role in folate metabolism, catalyzing the conversion of 5.10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cofactor for homocysteine remethylation to methionine. Genetic variants in the *MTHFR* gene, particularly C677T and A1298C polymorphisms, are linked to reduced enzyme activity, elevating homocysteine levels and potentially contributing to conditions like cardiovascular disease, neural tube defects, and psychiatric disorders. Antibodies targeting MTHFR are essential tools for studying its expression, localization, and functional alterations in research and diagnostics.
MTHFR antibodies are typically developed using recombinant human MTHFR protein or peptide epitopes as immunogens. They enable detection of MTHFR in tissues or cell lysates via techniques such as Western blotting, immunohistochemistry, or ELISA. These antibodies help assess protein levels in individuals with *MTHFR* mutations, clarify genotype-phenotype correlations, and evaluate the impact of epigenetic or environmental factors on enzyme expression. Commercial MTHFR antibodies are often validated for specificity across species, including human, mouse, and rat.
Clinically, MTHFR antibody-based assays may aid in diagnosing folate metabolism disorders or stratifying patients for personalized interventions, such as folate supplementation or homocysteine-lowering therapies. However, genetic testing remains the gold standard for identifying *MTHFR* variants, as antibody-based methods primarily address protein expression rather than specific mutations. Ongoing research focuses on optimizing antibody sensitivity and developing standardized protocols for translational applications.
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