| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MTH1; NUDT 1 |
| Entrez GeneID | 4521 |
| WB Predicted band size | Calculated MW: 18 kDa; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human MTH1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MTH1抗体的3篇参考文献，涵盖抗体开发、功能研究及疾病应用：
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1. **文献名称**：*"Selective targeting of cancer cells by a small molecule inhibitor of human MTH1"*
**作者**：Gad H. et al.
**摘要**：研究开发了一种MTH1小分子抑制剂，并利用MTH1抗体(Western blot和免疫荧光)验证其在癌细胞中的靶向性，证明抑制MTH1可诱导氧化损伤累积，选择性杀伤癌细胞。
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2. **文献名称**：*"Validation of a monoclonal antibody specific for human MTH1 protein by knockdown and immunohistochemical analysis"*
**作者**：Nakabeppu Y. et al.
**摘要**：报道了一种高特异性抗人MTH1单克隆抗体的开发与验证，通过siRNA敲低和免疫组化分析，证实该抗体可精准检测不同肿瘤组织中MTH1的表达水平，为癌症诊断提供工具。
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3. **文献名称**：*"MTH1 expression is required for cancer cell survival under oxidative stress in vitro and in vivo"*
**作者**：Huber K.V.M. et al.
**摘要**：利用MTH1抗体(免疫印迹和免疫组化)研究MTH1在氧化应激条件下的表达模式，发现其高表达与癌细胞存活显著相关，提示其作为治疗靶点的潜力。
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这些文献展示了MTH1抗体在靶向治疗开发、特异性验证及肿瘤生物学机制研究中的关键作用。
MTH1 (MutT homolog 1), also known as NUDT1. is a nucleotide pool sanitizing enzyme encoded by the *NUDT1* gene. It plays a critical role in maintaining genomic integrity by hydrolyzing oxidized nucleotides (e.g., 8-oxo-dGTP and 2-OH-dATP) to prevent their incorporation into DNA during replication. This activity is essential for reducing oxidative stress-induced DNA damage and mutations, which are linked to aging, neurodegeneration, and cancer.
MTH1 has gained significant attention in cancer research due to its overexpression in many tumors. Cancer cells often exhibit elevated oxidative stress, making them dependent on MTH1 to survive genomic instability. Consequently, MTH1 is considered a potential therapeutic target, with small-molecule inhibitors under investigation for anticancer therapy.
MTH1 antibodies are essential tools for studying the expression, localization, and function of MTH1 in cells and tissues. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies help researchers assess MTH1 levels in cancer versus normal tissues, evaluate its role in drug resistance, or validate MTH1 knockdown/knockout models. Specificity and validation (e.g., using knockout cell lines) are critical when selecting MTH1 antibodies to ensure accurate experimental results. Commercially available antibodies often target epitopes within the N-terminal or C-terminal regions of the protein.
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