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产品描述：人WNT1诱导信号通路蛋白1(WISP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WISP1含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外培养细胞、动物模型或人体来源样本的科研分析；可广泛用于探索WISP1在纤维化进程、肿瘤发生发展或代谢性疾病中的分子机制研究，为相关领域的基础科研提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCN4 ELISA Kit; WISP1 ELISA Kit; CCN family member 4 ELISA Kit; WNT1-inducible-signaling pathway protein 1 ELISA Kit; WISP-1 ELISA Kit; Wnt-1-induced secreted protein ELISA Kit
缩写：WISP1
Uniprot No.：O95388
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WISP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human WISP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.0582.1622.1101.937101.6541.6201.6371.46451.2411.2981.2701.0972.50.8340.8640.8490.6761.250.5120.5320.5220.3490.6250.3440.3610.3530.1800.3120.2420.2620.2520.07900.1720.1740.173 | ||||||
货期：3-5 working days
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