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产品描述：石斑鱼卵黄蛋白原(VTG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VTG含量。试剂盒检测范围为50 ng/mL-2000 ng/mL，适用于水产养殖中鱼类繁殖力评估、环境污染对生殖系统影响的机制研究，以及水生态毒理学领域的生物监测实验。为海洋生物生殖发育研究及生态毒理评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
缩写：VTG
Uniprot No.：Q5IGP8
种属：Grouper
样本类型：serum, plasma, tissue homogenates
检测范围：50 ng/mL-2000 ng/mL
灵敏度：50 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-103 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 20000.1740.1750.175 10000.3590.3780.369 4600.5670.5520.560 1500.8720.8910.882 501.5131.4761.495 02.3892.4112.400 | ||||||
货期：3-5 working days
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