首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人含V-set和Ig结构域蛋白4(VSIG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的VSIG4含量。VSIG4作为靶点，在免疫调节等方面具重要背景意义。其研究机制主要围绕免疫相关通路，它可与相关配体结合，调节免疫细胞功能，影响免疫反应进程，在肿瘤免疫、炎症疾病等研究领域受关注，有望为疾病治疗提供新靶点与策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中探究VSIG4在免疫微环境动态变化、疾病模型构建及药物干预效果评估中的作用。例如，在肿瘤免疫研究中可分析VSIG4与免疫检查点分子间的协同调控机制，在自身免疫性疾病模型中可监测巨噬细胞功能状态，或在炎症相关实验中评估组织特异性免疫抑制信号的变化趋势。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Complement receptor of the immunoglobulin superfamily ELISA Kit; CRIg ELISA Kit; Ig superfamily protein ELISA Kit; Protein Z39Ig ELISA Kit; UNQ317/PRO362 ELISA Kit; V set and immunoglobulin domain containing 4 ELISA Kit; V-set and immunoglobulin domain-containing protein 4 ELISA Kit; VSIG4 ELISA Kit; VSIG4_HUMAN ELISA Kit; Z39IG ELISA Kit
缩写：VSIG4
Uniprot No.：Q9Y279
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VSIG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human VSIG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-97 | ||||
| EDTA plasma (n=4) | 90 | 86-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.2982.1542.2262.035 10001.5571.5011.5291.338 5001.0251.0801.0530.862 2500.7000.6750.6880.497 1250.5100.4900.5000.309 62.50.3550.3490.3520.161 31.250.2360.2330.2350.044 00.1920.1890.191 | ||||||
货期：3-5 working days
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