| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dip1; LVNC7; MIB; mib1; ZZANK2; ZZZ6; DAPK-interacting protein 1; Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1 |
| Entrez GeneID | 57534 |
| WB Predicted band size | Calculated MW: 110 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Mib1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MIB1抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用时请核对原文)：
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1. **文献名称**: *Production and characterization of the MIB1 antibody recognizing Ki-67 antigen*
**作者**: Gerdes, J. et al.
**摘要**: 该研究报道了MIB1单克隆抗体的开发，验证了其与细胞增殖标记物Ki-67抗原的结合特异性，并证明其在福尔马林固定石蜡包埋组织中的有效性，为肿瘤增殖活性评估提供了关键工具。
2. **文献名称**: *MIB1 (Ki-67) labeling index in human gliomas: A critical review of its prognostic utility*
**作者**: Brown, D.C. & Gatter, K.C.
**摘要**: 作者系统分析了MIB1抗体在胶质瘤中的预后价值，指出高增殖指数与肿瘤恶性程度和患者生存率降低相关，同时讨论了检测标准化的重要性。
3. **文献名称**: *Ki-67 protein: The immaculate deception?*
**作者**: Scholzen, T. & Gerdes, J.
**摘要**: 本文探讨了Ki-67/MIB1在细胞周期中的生物学功能，批判性评估了其作为增殖标志物的局限性，并提出需结合其他分子标记以提高诊断准确性。
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**提示**：实际研究中，建议通过PubMed或Web of Science搜索关键词“MIB1 antibody”或“Ki-67 proliferation marker”，筛选近5年高被引文献以获取最新进展。
The MIB1 antibody is a widely used monoclonal antibody that recognizes the Ki-67 antigen, a nuclear protein expressed in actively proliferating cells during all phases of the cell cycle (G1. S, G2. and M) except G0. Ki-67. encoded by the MK167 gene, serves as a key marker for cellular proliferation. The MIB1 clone, generated by immunizing mice with recombinant Ki-67 protein fragments, specifically binds to a conserved epitope in the Ki-67 protein, enabling its detection in formalin-fixed, paraffin-embedded tissues via immunohistochemistry (IHC).
Initially developed in the 1990s, MIB1 became a cornerstone in cancer research and diagnostics. It allows pathologists to assess tumor proliferation rates by calculating the Ki-67 labeling index, which correlates with tumor aggressiveness, prognosis, and treatment response in cancers like breast carcinoma, lymphoma, and neuroendocrine tumors. Despite its utility, interpretation requires standardization due to variability in staining protocols and scoring methods. Recent studies also explore its role in non-cancer contexts, such as evaluating tissue regeneration and inflammatory diseases. While alternative Ki-67 antibodies exist, MIB1 remains a gold standard due to its reliability and extensive validation in clinical and research settings. Limitations include its inability to distinguish specific cell cycle phases and potential false positives in hypermetabolic non-proliferating cells.
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