首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B7-H4酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的VTCN1含量。VTCN1靶点它是重要的免疫调节分子，在肿瘤免疫逃逸中扮演角色。研究机制上，主要聚焦其与免疫细胞相互作用，通过调节免疫信号通路，影响机体免疫反应，目前相关研究致力于探索基于VTCN1的肿瘤免疫治疗新策略。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于肿瘤微环境研究、免疫调节机制探索等科研领域，尤其在评估肿瘤组织或炎症模型中 B7 - H4 的表达水平、筛选免疫检查点调控药物时具有重要应用价值，满足基础研究中对 B7 - H4 蛋白动态监测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B7 family member, H4 ELISA Kit; B7 H4 ELISA Kit; B7 homolog 4 ELISA Kit; B7 superfamily member 1 ELISA Kit; B7 superfamily, member 1 ELISA Kit; B7-H4 ELISA Kit; B7h.5 ELISA Kit; B7h4 ELISA Kit; B7S1 ELISA Kit; B7x ELISA Kit; BC032925 ELISA Kit; Immune costimulatory protein B7-H4 ELISA Kit; Immune costimulatory protein B7H4 ELISA Kit; MGC41287 ELISA Kit; PRO1291 ELISA Kit; Protein B7S1 ELISA Kit; RP11 229A19.4 ELISA Kit; T cell costimulatory molecule B7x ELISA Kit; T-cell costimulatory molecule B7x ELISA Kit; V set domain-containing T cell activation inhibitor 1 ELISA Kit; V-set domain-containing T-cell activation inhibitor 1 ELISA Kit; VCTN1 ELISA Kit; Vtcn1 ELISA Kit; VTCN1_HUMAN ELISA Kit
缩写：VTCN1
Uniprot No.：Q7Z7D3
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human B7-H4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-97 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of human B7-H4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-97 | ||||
| EDTA plasma (n=4) | 89 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.6252.7502.6882.5422502.1002.2742.1872.0411251.4661.4631.4651.31962.50.7590.7970.7780.63231.250.4750.4610.4680.32215.60.3620.3660.3640.2187.80.2310.2450.2380.09200.1420.1500.146 | ||||||
货期：3-5 working days
×
