| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MDH; MOR1; M-MDH; EIEE51; MGC:3559 |
| Entrez GeneID | 4191 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human MDH2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MDH2抗体的3篇参考文献的简要概括(文献为虚拟示例，仅供参考)：
1. **文献名称**：*"MDH2 Expression and Its Prognostic Significance in Colorectal Cancer"*
**作者**：Smith A, et al. (2018)
**摘要**：该研究通过免疫组化及Western blot分析，使用MDH2特异性抗体检测结直肠癌组织中MDH2蛋白表达水平，发现其表达下调与患者生存率降低相关，提示MDH2可能作为代谢重编程的生物标志物。
2. **文献名称**：*"Validation of a Novel Monoclonal MDH2 Antibody for Mitochondrial Studies"*
**作者**：Chen L, et al. (2021)
**摘要**：本文报道了一种新型MDH2单克隆抗体的开发与验证，通过质谱和敲除细胞系证实其高特异性，适用于免疫荧光及流式细胞术，为线粒体代谢研究提供了可靠工具。
3. **文献名称**：*"MDH2 Deficiency in Neuronal Cells Exacerbates Oxidative Stress"*
**作者**：Johnson R, et al. (2020)
**摘要**：研究利用MDH2抗体评估神经元中MDH2蛋白表达，发现其缺失导致线粒体活性氧积累，并阐明了MDH2在神经退行性疾病中的潜在保护机制。
(注：以上文献为示例性质，实际引用需以真实发表的学术论文为准。)
Malate dehydrogenase 2 (MDH2) is a mitochondrial enzyme central to the tricarboxylic acid (TCA) cycle, catalyzing the reversible oxidation of malate to oxaloacetate while reducing NAD⁺ to NADH. This reaction is critical for cellular energy production, redox balance, and metabolic intermediates for biosynthesis. Dysregulation of MDH2 has been linked to metabolic disorders, cancers, and neurodegenerative diseases, making it a research focus in mitochondrial dysfunction and cancer metabolism.
MDH2 antibodies are essential tools for detecting and quantifying MDH2 protein levels in studies exploring its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess tissue-specific expression, subcellular distribution, or alterations in disease models. Monoclonal antibodies offer high specificity, while polyclonal variants may detect conserved epitopes across species. Validated MDH2 antibodies aid in identifying its role in metabolic reprogramming (e.g., in tumors exploiting the TCA cycle for growth) or in mitochondrial disorders caused by genetic mutations. Researchers also utilize these antibodies to investigate post-translational modifications of MDH2 that regulate its activity. Proper validation via knockout controls or siRNA is crucial to ensure antibody reliability in experimental settings.
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