首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠维生素D3受体(VDR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VDR含量。VDR即维生素D受体，是核受体超家族成员。它能与维生素D结合后调节基因表达，在钙磷代谢、细胞增殖与分化、免疫调节等方面发挥重要作用。目前围绕VDR作用机制的研究不断深入，有望为相关疾病防治提供新策略。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于基础科研中探究VDR在骨骼代谢、免疫调控或疾病模型中的动态变化，例如维生素D信号通路机制研究、营养代谢相关动物实验或炎症性疾病模型分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vdr ELISA kit; Nr1i1Vitamin D3 receptor ELISA kit; VDR ELISA kit; 1,25-dihydroxyvitamin D3 receptor ELISA kit; Nuclear receptor subfamily 1 group I member 1 ELISA kit
缩写：VDR
Uniprot No.：P13053
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VDR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-105 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
回收率：
| The recovery of rat VDR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-89 | |||||
| EDTA plasma (n=4) | 95 | 91-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20001.9271.9581.9431.85910001.5371.5681.5531.4695001.1761.1311.1541.0702500.7890.7420.7660.6821250.4170.4010.4090.32562.50.2530.2640.2590.17531.20.1340.1380.1360.05200.0870.0810.084 | |||||||
货期：3-5 working days
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