| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LMNA; LMN1; Prelamin-A/C |
| Entrez GeneID | 4000 |
| WB Predicted band size | Calculated MW: 74 kDa; Observed MW: 74,63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Lamin A/C |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Lamin A/C抗体的3篇文献示例(内容为虚构模拟，仅作格式参考)：
1. **文献名称**：*Lamin A/C Antibody Specificity in Nuclear Envelope Staining*
**作者**：Karen L. Reddy et al.
**摘要**：本研究验证了多种Lamin A/C抗体在免疫荧光和Western blot中的特异性，发现部分抗体与Lamin B存在交叉反应，推荐使用克隆号4C7的抗体以减少假阳性结果。
2. **文献名称**：*Role of Lamin A/C Mutations in Cardiomyopathy: Antibody-Based Detection*
**作者**：Brian Burke et al.
**摘要**：通过Lamin A/C抗体分析心肌病患者组织样本，发现突变导致核纤层结构异常，抗体染色显示核形态畸变与疾病严重程度相关。
3. **文献名称**：*Lamin A/C Antibody Applications in Progeria Research*
**作者**：Tom Misteli et al.
**摘要**：利用Lamin A/C抗体研究早衰症细胞模型，揭示了截短型progerin蛋白的异常定位，抗体染色证实其干扰正常核纤层组装。
4. **文献名称**：*Lamin A/C as a Biomarker in Cancer: Comparative Antibody Analysis*
**作者**：Paola Scaffidi et al.
**摘要**：比较不同Lamin A/C抗体在肿瘤组织中的染色效果，发现Lamin A/C表达缺失与乳腺癌转移相关，强调抗体选择对临床样本分析的重要性。
(注：以上文献为示例，实际引用需检索PubMed等数据库获取真实研究。)
Lamin A/C antibodies are essential tools in studying nuclear architecture and associated diseases. Lamin A and C, encoded by the *LMNA* gene, are key components of the nuclear lamina—a mesh-like structure underlying the inner nuclear membrane. They play critical roles in maintaining nuclear integrity, regulating chromatin organization, and influencing gene expression. As type V intermediate filaments, lamin A/C undergoes post-translational processing, with lamin A requiring cleavage of a C-terminal farnesyl group for maturation, while lamin C lacks this modification.
Mutations in *LMNA* are linked to diverse disorders termed laminopathies, including Hutchinson-Gilford progeria syndrome (HGPS), muscular dystrophies, and cardiomyopathy. These mutations disrupt nuclear mechanics, DNA repair, and cellular signaling. Antibodies targeting lamin A/C are widely used in research to investigate nuclear morphology, subcellular localization, and disease mechanisms. They enable detection via techniques like immunofluorescence, Western blotting, and immunohistochemistry. Notably, some antibodies distinguish lamin A from lamin C by recognizing unique C-terminal epitopes. In diagnostics, lamin A/C antibodies aid in identifying nuclear abnormalities in laminopathy patient samples or cancer cells, where altered lamin expression correlates with metastatic potential. Recent studies also explore lamin A/C's role in aging, stem cell differentiation, and viral infection responses, highlighting their broader biological relevance.
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