| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LMN2; LAMB2 |
| Entrez GeneID | 84823 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Lamin B2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Lamin B2抗体的3篇参考文献示例(文献信息为模拟内容，实际引用请核实原文)：
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1. **文献名称**: *Lamin B2 regulates mitotic spindle assembly during somatic cell division*
**作者**: Smith J, et al.
**摘要**: 本研究通过免疫荧光和Western blot技术，利用Lamin B2抗体揭示了Lamin B2在小鼠成纤维细胞有丝分裂中对纺锤体组装的调控作用。结果表明，Lamin B2的缺失会导致染色体排列异常和细胞周期停滞。
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2. **文献名称**: *Downregulation of Lamin B2 in glioblastoma promotes tumor invasion*
**作者**: Chen L, et al.
**摘要**: 该研究通过免疫组织化学(IHC)和免疫印迹分析，发现Lamin B2在胶质母细胞瘤中表达显著下调，并与患者预后不良相关。实验表明，Lamin B2抗体检测到的低表达水平可能通过破坏核膜完整性促进肿瘤细胞侵袭。
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3. **文献名称**: *Lamin B2 deficiency induces premature senescence in human fibroblasts*
**作者**: Wang Y, et al.
**摘要**: 利用CRISPR-Cas9敲除Lamin B2并结合抗体验证蛋白表达，本研究发现Lamin B2缺失会导致人类原代成纤维细胞早衰，伴随核形态异常和衰老相关分泌表型(SASP)激活，提示其在细胞衰老中的关键作用。
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(注：上述文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。)
Lamin B2 is a key component of the nuclear lamina, a structural meshwork underlying the inner nuclear membrane. It belongs to the B-type lamin family, which includes Lamin B1 and B3. and plays critical roles in maintaining nuclear integrity, chromatin organization, and gene regulation. Unlike A-type lamins, Lamin B2 is constitutively expressed in most somatic cells and is essential for embryonic development. Its antibodies are widely used to study nuclear architecture, cell cycle dynamics, and diseases linked to nuclear envelope dysfunction, such as cancer, premature aging syndromes (e.g., progeria), and neurodegenerative disorders.
Anti-Lamin B2 antibodies are valuable tools for detecting protein localization via immunofluorescence, quantifying expression levels through Western blotting, or isolating nuclei in cell fractionation experiments. They help distinguish B-type lamins from A-type isoforms (e.g., Lamin A/C), aiding research on lamin-specific functions. Commercial antibodies are typically raised against conserved epitopes, often validated for cross-reactivity in human, mouse, and rat models. Studies using these antibodies have revealed Lamin B2’s involvement in mitotic spindle formation, DNA repair, and cellular senescence. Recent work also explores its role in cancer metastasis, where altered Lamin B2 expression correlates with tumor aggressiveness. Proper validation, including knockout controls, is crucial due to potential cross-reactivity with other lamins.
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