首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人维生素D受体(VD R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的VDR含量。VDR即维生素D受体，在人体多个组织器官广泛分布。它能与活性维生素D结合形成复合物，调控相关基因表达。其研究机制主要围绕调节钙磷代谢、细胞增殖分化及免疫功能等方面，对骨骼健康、肿瘤防治等领域研究意义重大。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于生命科学领域研究，可用于探索维生素D代谢调控机制、骨代谢疾病模型构建、免疫相关信号通路分析等实验场景，支持体外样本中VDR蛋白的定量检测需求，为科研人员提供高效可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit
缩写：VDR
Uniprot No.：P11473
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-94 | ||||
| EDTA plasma (n=4) | 99 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.1782.0982.1381.9772001.5621.6441.6031.4421001.1381.1181.1280.967500.8110.8030.8070.646250.4520.4600.4560.29512.50.3250.3150.3200.1596.250.2270.2310.2290.06800.1620.1600.161 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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