首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人过渡性内质网ATP酶(VCP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的VCP含量。VCP是一种多功能ATP酶，在细胞内蛋白质稳态、自噬等多种生理过程中发挥关键作用。其异常与多种神经退行性疾病、癌症等密切相关。研究机制集中于其ATP酶活性调节、与底物及共因子相互作用，以探寻干预疾病的潜在靶点。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于基础科研中探索VCP在细胞应激反应、蛋白质稳态调控等领域的分子机制。兼容多种生物样本类型的特点使其可广泛应用于体外培养细胞模型、动物组织样本或体液来源样本的VCP表达水平分析，为研究疾病相关蛋白质代谢异常、药物干预效果评估等提供可靠工具，特别适用于神经生物学、肿瘤微环境及分子病理学等领域的定量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：15S Mg(2+) ATPase p97 subunit ELISA Kit; 15S Mg(2+)-ATPase p97 subunit ELISA Kit; ALS14 ELISA Kit; ATPase p97 ELISA Kit; CDC48 ELISA Kit; IBMPFD ELISA Kit; MGC131997 ELISA Kit; MGC148092 ELISA Kit; MGC8560 ELISA Kit; p97 ELISA Kit; TER ATPase ELISA Kit; TERA ELISA Kit; TERA_HUMAN ELISA Kit; Transitional endoplasmic reticulum ATPase ELISA Kit; Valosin containing protein ELISA Kit; Valosin-containing protein ELISA Kit; VCP ELISA Kit; Yeast Cdc48p homolog ELISA Kit
缩写：VCP
Uniprot No.：P55072
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 96-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 88-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-95 | |||||
回收率：
| The recovery of human VCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 103 | 97-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.0722.0872.0801.9137501.4041.4241.4141.2473750.8120.8230.8180.651187.50.4660.4480.4570.29093.750.3360.3450.3410.17446.880.2720.2660.2690.10223.440.2260.2170.2220.05500.1660.1680.167 | ||||||
货期：3-5 working days
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