首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人黏着斑蛋白(VCL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的VCL含量。VCL即Vinculin，是一种与细胞黏附相关的蛋白质。它在维持细胞形态、调控细胞迁移等方面发挥重要作用。其作用机制主要是通过与肌动蛋白等相互作用，介导细胞与细胞、细胞与基质间的连接，目前在肿瘤转移等研究领域受关注。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于细胞生物学研究中细胞黏附机制探索、肿瘤转移模型分析、心血管疾病相关细胞力学研究，以及组织修复或纤维化过程中细胞外基质互作的分子机制解析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CMD1W ELISA Kit; CMH15 ELISA Kit; Epididymis luminal protein 114 ELISA Kit; HEL114 ELISA Kit; Metavinculin ELISA Kit; MV ELISA Kit; MVCL ELISA Kit; OTTHUMP00000019861 ELISA Kit; OTTHUMP00000019862 ELISA Kit; VCL ELISA Kit; VINC ELISA Kit; VINC_HUMAN ELISA Kit; Vinculin ELISA Kit
缩写：VCL
Uniprot No.：P18206
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VCL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-104 | |||||
回收率：
| The recovery of human VCL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.0432.1522.0981.9599001.4411.3421.3921.2534500.7820.8240.8030.6642250.4630.4920.4780.3391120.3310.3240.3280.189560.2470.2340.2410.102280.1890.1840.1870.04800.1380.1400.139 | ||||||
货期：3-5 working days
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