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产品描述：猪血管内皮细胞粘附分子1(VCAM-1/CD106)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VCAM1含量。VCAM1（血管细胞粘附分子1）是一种细胞表面糖蛋白，属于免疫球蛋白超家族，主要表达于活化的内皮细胞表面。它通过介导白细胞与内皮细胞的粘附，在免疫反应和炎症过程中发挥重要作用。研究显示，VCAM1在多种疾病中扮演关键角色，如动脉粥样硬化、炎症性肠病和癌症，其表达水平与疾病进展密切相关。试剂盒检测范围为15.6ng/mL-1000ng/mL，适用于研究猪心血管疾病模型中的内皮细胞活化机制、炎症性疾病病理进程评估，以及免疫调节药物对血管内皮功能影响的体外实验，为探索VCAM - 1在血管相关疾病中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：VCAM1/CD106
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6ng/mL-1000ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 95-104 | |||||
回收率：
| The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 89 | 83-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.3352.1782.2572.1005001.5741.4751.5251.3682501.0070.9820.9950.8381250.5910.5860.5890.43262.50.3860.3940.3900.23331.20.2780.2820.2800.12315.60.2280.2250.2270.07000.1580.1560.157 | ||||||
货期：3-5 working days
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