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产品描述：大鼠血管内皮细胞粘附分子1(VCAM-1/CD106)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VCAM1含量。VCAM1，即血管细胞粘附分子1，是一种免疫球蛋白超家族成员，主要表达于活化的血管内皮细胞表面。它通过与白细胞表面的特定分子结合，介导白细胞与内皮细胞的粘附，参与炎症反应、免疫应答、淋巴细胞归巢等生理过程。研究显示，VCAM1在衰老相关疾病如阿尔茨海默病中发挥重要作用，其表达水平与大脑功能衰退密切相关。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于炎症机制研究、心血管疾病动物模型评估、药物干预效果观察等科研场景，为探索内皮细胞活化、白细胞迁移等生物学过程提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit
缩写：VCAM-1/CD106
Uniprot No.：P29534
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 86-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 82-105 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 87-105 | |||||
回收率：
| The recovery of rat VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-104 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.6142.7042.6592.5255002.1452.2362.1912.0572501.5161.5361.5261.3921250.8630.8480.8560.72262.50.5710.5490.5600.42631.250.3520.3470.3500.21615.60.2340.2440.2390.10500.1350.1320.134 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat VCAM antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled VCAM antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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