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产品描述：人蛋白基因产物9.5(PGP 9.5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的UCHL1含量。UCHL1 是一个重要靶点。它在神经系统中高表达，与神经元的存活和功能维持相关。研究机制方面，主要聚焦其在泛素-蛋白酶体系统中的作用，UCHL1 可调控泛素代谢，其异常表达或功能改变会影响多种疾病发生发展，如神经系统疾病。试剂盒检测范围为6.25 ng/mL-400 ng/mL，该产品适用于神经生物学领域的基础研究，例如通过脑脊液检测探索中枢神经系统疾病模型中的神经损伤标志物变化，或通过组织匀浆分析神经退行性疾病（如阿尔茨海默病、帕金森病）动物模型的蛋白表达特征，也可用于神经内分泌肿瘤细胞系或外周血样本的神经活性物质研究，为神经发育、轴突再生及病理机制提供定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epididymis luminal protein 117 ELISA Kit; Epididymis secretory protein Li 53 ELISA Kit; HEL 117 ELISA Kit; HEL S 53 ELISA Kit; NDGOA ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; OTTHUMP00000218137 ELISA Kit; OTTHUMP00000218139 ELISA Kit; OTTHUMP00000218140 ELISA Kit; OTTHUMP00000218141 ELISA Kit; Park 5 ELISA Kit; PARK5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; PGP95 ELISA Kit; Protein gene product 9.5 ELISA Kit; Ubiquitin C terminal esterase L1 ELISA Kit; Ubiquitin C terminal hydrolase ELISA Kit; Ubiquitin C terminal hydrolase L1 ELISA Kit; Ubiquitin carboxyl terminal esterase L1 ELISA Kit; Ubiquitin carboxyl terminal hydrolase isozyme L1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit; Ubiquitin thiolesterase ELISA Kit; Ubiquitin thiolesterase L1 ELISA Kit; UCH-L1 ELISA Kit; UCHL1 ELISA Kit; UCHL1_HUMAN ELISA Kit
缩写：UCHL1
Uniprot No.：P09936
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGP 9.5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human PGP 9.5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-101 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.2692.3892.3292.2352001.7781.7981.7881.6941001.2411.2691.2551.161500.7260.7580.7420.648250.4140.4360.4250.33112.50.2710.2550.2630.1696.250.1650.1690.1670.07300.0920.0950.094 | ||||||
货期：3-5 working days
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