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产品描述：犬促甲状腺素(TSH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSH含量。促甲状腺激素（TSH）由腺垂体分泌，主要负责调节甲状腺细胞的生长和甲状腺激素的合成与释放，维持甲状腺的正常功能。对其研究聚焦于其与下丘脑 - 垂体 - 甲状腺轴的反馈调节机制，以及在甲状腺疾病诊断和治疗中的应用。试剂盒检测范围为0.625 μIU/mL-10 μIU/mL，该产品兼容多种生物样本类型，尤其适用于科研场景中甲状腺功能机制研究、药物对内分泌系统影响的评估，或疾病模型动物的激素水平动态监测，为兽医科研、宠物营养学及内分泌相关基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
缩写：TSH
Uniprot No.：P54828
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μIU/mL-10 μIU/mL
灵敏度：0.625 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 82 | ||||
| Range % | 80-84 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of canine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 100.1950.1880.192 50.3340.3530.344 2.50.5310.5140.523 1.251.0171.1021.060 0.6252.1382.3202.229 | ||||||
货期：3-5 working days
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