首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肿瘤坏死因子配体超家族成员9(TNFSF9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF9含量。TNFSF9 属于肿瘤坏死因子超家族成员，在免疫调节等生理过程中发挥重要作用。其研究机制方面，主要聚焦于通过与受体相互作用激活下游信号通路，在肿瘤免疫疗法中作为潜在靶点，可增强免疫细胞对肿瘤细胞的杀伤作用。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品可广泛应用于免疫调控机制研究、肿瘤微环境分析、炎症性疾病模型构建等基础科研领域，为体外探究TNFSF9在疾病发生发展中的生物学功能及潜在干预靶点提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4 1BB L ELISA Kit; 4 1BB ligand ELISA Kit; 4 1BBL ELISA Kit; 4-1BB ligand ELISA Kit; 4-1BBL ELISA Kit; Cd137l ELISA Kit; Cd157l ELISA Kit; Homolog of mouse 4 1BB L ELISA Kit; Homolog of mouse 4 1BBL ELISA Kit; ILA ligand (TNF related) ELISA Kit; Ly63l ELISA Kit; Receptor 4 1BB ligand ELISA Kit; TNF superfamily member 9 ELISA Kit; TNFL9_HUMAN ELISA Kit; Tnfsf9 ELISA Kit; TNLG5A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 9 ELISA Kit; Tumor necrosis factor ligand 5A ELISA Kit; Tumor necrosis factor ligand superfamily member 9 ELISA Kit; Tumor necrosis factor superfamily member 9 ELISA Kit
缩写：TNFSF9
Uniprot No.：P41273
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNFSF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human TNFSF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-108 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.0211.9151.9681.874 6001.3041.3861.3451.251 3000.8740.8600.8670.773 1500.5750.5600.5680.474 750.4000.3820.3910.297 37.50.2750.2710.2730.179 18.750.1800.1760.1780.084 00.0940.0930.094 | ||||||
货期：3-5 working days
×
