| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCN1; CCNA |
| Entrez GeneID | 890 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Cyclin A2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于Cyclin A2抗体的参考文献示例(文献信息为模拟概括，仅供参考)：
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1. **文献名称**: *"Cyclin A is required for the onset of DNA replication in mammalian fibroblasts"*
**作者**: Pagano M, et al.
**摘要**: 该研究利用Cyclin A2特异性抗体，通过免疫沉淀和Western blot分析，揭示了Cyclin A2在哺乳动物细胞DNA复制起始中的关键作用，并证明其表达水平与细胞周期S期进程密切相关。
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2. **文献名称**: *"Distinct roles for cyclin A2 in cancer progression and therapeutic resistance"*
**作者**: Malumbres M, et al.
**摘要**: 文章通过免疫组化(IHC)和流式细胞术结合Cyclin A2抗体，探讨了Cyclin A2在多种癌症中的异常表达及其与肿瘤增殖、转移和化疗耐药性的关联，为靶向治疗提供依据。
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3. **文献名称**: *"Antibody-based detection of Cyclin A2 in cell cycle studies"*
**作者**: Yam CH, et al.
**摘要**: 该研究系统评估了多种Cyclin A2抗体的特异性及适用场景，比较了其在Western blot、免疫荧光(IF)和流式细胞术中的表现，为实验中选择抗体提供技术参考。
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4. **文献名称**: *"Cyclin A2 degradation regulates exit from S phase in human cells"*
**作者**: Wolthuis R, et al.
**摘要**: 通过Cyclin A2抗体追踪蛋白动态，研究发现泛素-蛋白酶体系统介导的Cyclin A2降解是细胞退出S期并进入G2/M期的必要条件，揭示了细胞周期调控的分子机制。
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注：以上文献名称及内容为示例性概括，实际文献需通过PubMed或学术数据库检索确认。
Cyclin A2 is a critical regulatory protein involved in cell cycle progression, primarily functioning during the S phase and G2/M transition. As a member of the cyclin family, it binds to and activates cyclin-dependent kinases (CDKs), particularly CDK2 and CDK1. to drive DNA replication and mitotic entry. Its expression is tightly regulated, peaking during S phase and declining after mitosis. Dysregulation of Cyclin A2 is associated with various cancers, where its overexpression often correlates with uncontrolled proliferation, genomic instability, and poor clinical outcomes.
Antibodies targeting Cyclin A2 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Cyclin A2 levels in cell lines, tissues, or clinical samples. Specificity is a key consideration, as Cyclin A2 shares structural homology with other cyclins (e.g., Cyclin A1). High-quality antibodies are validated using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity. In cancer research, Cyclin A2 antibodies help evaluate proliferative activity, tumor grade, and therapeutic responses. Additionally, they contribute to mechanistic studies exploring cell cycle checkpoints, drug targets, and pathways linked to carcinogenesis. Reliable Cyclin A2 antibodies thus serve as vital reagents for advancing understanding of cell cycle dynamics and developing diagnostic or therapeutic strategies.
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