| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ccn1; Ccna; Cyca; Ccn-1; CycA2; AA408589 |
| Entrez GeneID | 12428 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of mouse Cyclin A2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclin A2抗体的3-4篇参考文献，包含文献名称、作者及简要摘要内容：
1. **"Cyclin A is required at two points in the human cell cycle"**
- **作者**: Pagano, M. et al.
- **摘要**: 该研究通过Cyclin A2特异性抗体证实Cyclin A2在S期和G2/M期的关键作用，揭示其通过结合CDK2调控DNA复制和有丝分裂进程的机制。
2. **"Cyclin A2 regulates DNA damage repair and cancer progression"**
- **作者**: Huang, H. et al.
- **摘要**: 文章利用Cyclin A2抗体进行免疫沉淀实验，发现Cyclin A2过表达与多种癌症预后不良相关，并参与调控DNA损伤修复通路，可能成为潜在治疗靶点。
3. **"Characterization of monoclonal antibodies specific for Cyclin A2"**
- **作者**: Woo, R.A. & Poon, R.Y.
- **摘要**: 该研究系统评估了不同Cyclin A2抗体的特异性及适用场景，验证了其在免疫印迹、免疫荧光等实验中的可靠性，为相关研究提供抗体选择依据。
4. **"Cyclin A2-CDK activity underlies replication stress response"**
- **作者**: Machida, Y.J. & Dutta, A.
- **摘要**: 通过Cyclin A2抗体阻断实验，阐明Cyclin A2-CDK复合物在复制应激中稳定停滞的复制叉并促进修复的分子机制。
以上文献均涉及Cyclin A2的功能研究及抗体应用，涵盖细胞周期调控、癌症及实验技术方向。建议通过PubMed或期刊官网以标题/作者进一步检索全文。
Cyclin A2 is a critical regulatory protein involved in cell cycle progression, primarily functioning during the S phase and G2/M phase transition. It forms complexes with cyclin-dependent kinases (CDKs), such as CDK2 and CDK1. to drive DNA replication and mitosis by phosphorylating target substrates. Dysregulation of Cyclin A2 is linked to uncontrolled cell proliferation, tumorigenesis, and poor prognosis in various cancers, making it a biomarker of interest in oncology research.
Antibodies against Cyclin A2 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies enable detection via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity is validated through knockdown/knockout controls or peptide-blocking assays to ensure accurate identification of Cyclin A2 isoforms, which share homology with other cyclins (e.g., Cyclin A1). Researchers use these antibodies to explore Cyclin A2's roles in cell cycle checkpoints, DNA damage responses, and its potential as a therapeutic target. Commercial antibodies are typically raised in rabbits or mice, with clones optimized for distinct applications. Beyond cancer, Cyclin A2 antibodies also contribute to studies in developmental biology, cardiovascular diseases, and neurodegenerative disorders, reflecting its broad impact on cellular homeostasis.
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