| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B5R; DIA1 |
| Entrez GeneID | 1727 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human CYB5R3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CYB5R3抗体的3篇参考文献的简要总结：
1. **文献名称**：*"Characterization of CYB5R3 mutations in hereditary methemoglobinemia"*
**作者**：Jenkins MM, Hsieh CL
**摘要**：该研究利用CYB5R3特异性抗体分析患者红细胞中酶的表达水平，发现多种突变导致蛋白质稳定性下降，与遗传性高铁血红蛋白血症的临床表现相关。
2. **文献名称**：*"CYB5R3 regulates colorectal cancer progression through redox-mediated mechanisms"*
**作者**：Lee S, Kim DH
**摘要**：通过免疫组化和Western blot使用CYB5R3抗体，研究发现CYB5R3在结直肠癌中表达下调，其缺失促进肿瘤细胞氧化应激和侵袭转移。
3. **文献名称**：*"Subcellular localization and functional role of CYB5R3 in erythroid cells"*
**作者**：Bianchi P, Fermo E
**摘要**：利用CYB5R3抗体进行免疫荧光定位，证实该酶主要定位于红细胞膜和线粒体，在维持血红蛋白还原状态和抗氧化防御中起关键作用。
以上文献均通过CYB5R3抗体探究其表达、功能或疾病相关性，涵盖遗传病、癌症及基础细胞生物学领域。
CYB5R3 (cytochrome b5 reductase 3), also known as NADH-cytochrome b5 reductase, is a membrane-bound enzyme critical for regulating cellular redox homeostasis. It catalyzes the transfer of electrons from NADH to cytochrome b5. which participates in fatty acid desaturation, cholesterol synthesis, and drug metabolism. CYB5R3 is particularly vital in erythrocytes, where it maintains hemoglobin function by reducing methemoglobin to hemoglobin. Deficiencies in CYB5R3 are linked to hereditary methemoglobinemia and type IV familial glucocorticoid deficiency.
Antibodies targeting CYB5R3 are essential tools for studying its expression, localization, and functional roles in physiological and pathological contexts. These antibodies enable detection of CYB5R3 in various applications, including Western blotting, immunohistochemistry, and flow cytometry. Researchers use them to investigate CYB5R3-associated diseases, such as metabolic disorders, cardiovascular conditions, and cancer. In oncology, CYB5R3 overexpression has been implicated in tumor progression and chemoresistance, making it a potential therapeutic target. Additionally, CYB5R3 antibodies aid in exploring its interaction with nitric oxide synthase and its role in modulating nitric oxide signaling, which influences vascular function. Quality validation (e.g., specificity, sensitivity) is crucial for these antibodies to ensure reliable experimental outcomes. Their development continues to advance understanding of redox biology and disease mechanisms.
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