首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可溶性OX40L(sOX40L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF4含量。TNFSF4是一个重要靶点。它在免疫调节等方面有重要作用，参与多种免疫相关疾病的发生发展。其研究机制主要围绕与受体相互作用，调控免疫细胞的活化、增殖与功能，为开发针对免疫疾病的治疗方法提供了关键理论基础。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于免疫调控机制研究、疾病模型评估或生物标志物探索等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNFSF4; TXGP1; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD antigen CD252
缩写：TNFSF4
Uniprot No.：P23510
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sOX40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 106 | ||||
| Range % | 100-113 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 82-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human sOX40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5542.6032.5792.45051.9752.0652.0201.8912.51.3051.4021.3541.2251.250.7440.7140.7290.6000.6250.4760.4980.4870.3580.3120.3510.3260.3390.2100.1560.2270.2260.2270.09800.1320.1260.129 | ||||||
货期：3-5 working days
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