| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCN1; CCNA |
| Entrez GeneID | 890 |
| WB Predicted band size | Calculated MW: 49 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Cyclin A2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于Cyclin A2抗体的参考文献(示例仅供参考，建议根据实际文献调整)：
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1. **文献名称**: *"Cyclin A2 regulates nuclear envelope breakdown and the nuclear accumulation of cyclin B1"*
**作者**: Kossatz U. et al.
**摘要**: 研究利用Cyclin A2抗体通过免疫荧光和Western blot分析，揭示了Cyclin A2在有丝分裂早期通过调控核膜分解影响Cyclin B1核定位的机制。
2. **文献名称**: *"Cyclin A2 expression in hepatocellular carcinoma: Correlation with clinicopathological features and prognosis"*
**作者**: Yuan J. et al.
**摘要**: 通过免疫组化(使用Cyclin A2特异性抗体)分析肝细胞癌组织中Cyclin A2的表达水平，发现其过表达与肿瘤进展和不良预后显著相关。
3. **文献名称**: *"Distinct roles for cyclin A2 in DNA replication and mitotic entry"*
**作者**: Pagano M. et al.
**摘要**: 研究采用Cyclin A2抗体进行流式细胞术和免疫沉淀，证明Cyclin A2通过调控S期DNA复制和有丝分裂进入，维持细胞周期时序性。
4. **文献名称**: *"Antibody-based validation of Cyclin A2 degradation during mitotic exit"*
**作者**: Pines J. et al.
**摘要**: 通过Western blot和免疫荧光(基于Cyclin A2单克隆抗体)，证实Cyclin A2在有丝分裂退出阶段通过泛素-蛋白酶体途径降解的分子机制。
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**注**：以上为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索。推荐使用关键词“Cyclin A2 antibody”或“Cyclin A2 function”筛选近年高影响力论文。
Cyclin A2 is a key regulatory protein involved in cell cycle progression, primarily functioning during the S and G2/M phases. It forms complexes with cyclin-dependent kinases (CDKs), particularly CDK1 and CDK2. to drive DNA replication and mitotic entry. As a critical checkpoint controller, Cyclin A2 ensures proper timing and coordination of cell division, making it essential for cellular proliferation and genome stability. Dysregulation of Cyclin A2 expression is closely linked to various cancers, where its overexpression correlates with aggressive tumor behavior and poor prognosis. It has also been implicated in cardiovascular diseases, such as cardiac hypertrophy, due to its role in cell cycle re-entry in terminally differentiated cells.
Antibodies targeting Cyclin A2 are widely used in research to study cell cycle dynamics, cancer mechanisms, and therapeutic responses. These antibodies enable detection and quantification of Cyclin A2 protein levels via techniques like Western blotting, immunohistochemistry, and flow cytometry. Specific applications include assessing proliferation rates in tumors, evaluating cell cycle arrest in drug development, and exploring tissue regeneration pathways. Commercial Cyclin A2 antibodies are typically validated for specificity across human, mouse, and rat models. Recent studies also investigate its potential as a diagnostic marker or target for anticancer therapies, particularly in malignancies like hepatocellular carcinoma and breast cancer. Proper antibody validation remains crucial to avoid cross-reactivity with other cyclins, such as Cyclin A1 or B1.
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