Mouse soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA Kit

产品详情

中文名： 小鼠可溶性核因子κB受体活化因子配基(sRANKL)ELISA Kit

货号: IPD-E05127m

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
小鼠可溶性核因子κB受体活化因子配基(sRANKL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的TNFSF11含量。TNFSF11是重要靶点，它属于肿瘤坏死因子超家族，与骨代谢密切相关，参与破骨细胞的分化、活化和存活调节。在相关疾病研究中，围绕其与骨质疏松、骨肿瘤等发病机制的关系及阻断该靶点以治疗疾病的机制是研究热点。试剂盒检测范围为12.5 pg/ml-800 pg/ml.，适用于骨代谢疾病模型研究、炎症信号通路分析或免疫调节相关实验，为探索sRANKL在骨组织重建、免疫细胞活化等生物学过程中的作用机制提供可靠工具。支持科研人员高效开展细胞实验、动物模型或离体组织样本的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tnfsf11 ELISA Kit; Opgl ELISA Kit; Rankl ELISA Kit; Trance ELISA Kit; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; Osteoclast differentiation factor ELISA Kit; ODF ELISA Kit; Osteoprotegerin ligand ELISA Kit; OPGL ELISA Kit; Receptor activator of nuclear factor kappa-B ligand ELISA Kit; RANKL ELISA Kit; TNF-related activation-induced cytokine ELISA Kit; TRANCE ELISA Kit; CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11 ELISA Kit; membrane form; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; soluble form] ELISA Kit
缩写：
TNFSF11
Uniprot No.：
O35235
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
12.5 pg/ml-800 pg/ml.
灵敏度：
3.12 pg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sRANKL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-105
1:2	Average %	97
	Range %	91-105
1:4	Average %	97
	Range %	92-106
1:8	Average %	94
	Range %	89-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
800	2.549	2.634	2.591	2.442
400	1.774	1.667	1.721	1.571
200	0.927	0.948	0.937	0.788
100	0.597	0.609	0.603	0.453
50	0.341	0.321	0.331	0.181
25	0.254	0.253	0.254	0.104
12.5	0.194	0.184	0.189	0.039
0	0.154	0.145	0.150