| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLN80; COIL; Coilin; Coilin p80 |
| Entrez GeneID | 8161 |
| WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Coilin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Coilin抗体的3篇参考文献(虚构示例，仅供参考)：
1. **文献名称**：*Coilin: A Cajal Body Marker Protein with Dynamic Nuclear Localization*
**作者**：Andrade, E.C., et al.
**摘要**：该研究通过免疫荧光和Western blot技术验证了多种Coilin抗体的特异性，发现Coilin在HeLa细胞核中的定位与卡哈尔体(Cajal bodies)密切相关，并探讨了其在RNA剪接复合体组装中的作用。
2. **文献名称**：*Characterization of Anti-Coilin Antibodies in Autoimmune Disorders*
**作者**：Raska, I., et al.
**摘要**：文章分析了来自不同物种的Coilin抗体在自身免疫性疾病患者血清中的交叉反应性，发现部分抗体可特异性识别Coilin的C端结构域，并揭示了其与核小体结构的潜在关联。
3. **文献名称**：*Coilin Depletion Alters snRNP Biogenesis: Insights from Antibody-Mediated Knockdown*
**作者**：Hebert, M.D., & Matera, A.G.
**摘要**：研究利用Coilin抗体进行免疫沉淀和功能抑制实验，证明Coilin缺失影响小核核糖核蛋白(snRNP)的成熟，并揭示了其在细胞应激反应中的调控机制。
(注：以上文献为示例，实际引用需根据真实研究调整。)
Coilin antibodies are essential tools in studying the structure and function of Cajal bodies (CBs), membrane-less subnuclear organelles involved in RNA processing and ribonucleoprotein (RNP) assembly. Coilin, a 62-64 kDa phosphoprotein, is a defining marker of CBs and plays a scaffolding role in their formation and maintenance. First identified in the 1990s, coilin is critical for the biogenesis of small nuclear RNPs (snRNPs), telomerase assembly, and histone mRNA processing. Its dynamic interactions with components like SMN (survival of motor neuron) protein link CBs to neurodegenerative diseases, particularly spinal muscular atrophy (SMA).
Coilin antibodies, typically generated against recombinant or peptide-derived coilin, enable the detection and localization of CBs via techniques like immunofluorescence, Western blotting, and immunoprecipitation. These antibodies have revealed CB heterogeneity across cell types and their disassembly during mitosis. In disease research, altered CB dynamics—observed using coilin antibodies—are associated with cancer, viral infections, and neurological disorders. For instance, reduced CB integrity in SMA models correlates with snRNP biogenesis defects. Commercially available coilin antibodies (e.g., from Santa Cruz, Abcam) vary in specificity, requiring validation for experimental conditions. Recent advances in super-resolution microscopy further leverage these antibodies to explore CB ultrastructure. Ongoing studies focus on coilin’s post-translational modifications (e.g., phosphorylation) and their regulatory roles, underscoring the antibody’s continued relevance in cell biology and translational research.
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