首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可溶性核因子κB受体活化因子配基(sRANKL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、cellculturesupeernates、组织匀浆样本中的TNFSF11含量。TNFSF11是重要靶点。它参与骨代谢调节等生理过程。研究上，聚焦其在骨相关疾病机制，如破骨细胞形成与活化机制。其信号通路与骨质疏松等疾病发病密切相关，以此为靶点研发药物对骨病治疗有重要意义。试剂盒检测范围为7.8 pg/mL-500 pg/mL，为研究骨稳态失衡机制、炎症性疾病病理进程以及相关药物干预效果评价提供可靠工具，尤其适用于体外模型中的蛋白表达水平监测或生物样本中sRANKL动态变化的定量分析，为分子机制探索及生物标志物研究提供精准数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNFSF11; OPGL; RANKL; TRANCE; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine; CD antigen CD254
缩写：TNFSF11
Uniprot No.：O14788
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supeernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sRANKL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:10 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human sRANKL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8032.7522.7782.6082502.2432.1762.2102.0401251.4651.4041.4351.26562.50.8910.8390.8650.69531.20.4720.4510.4620.29215.60.3640.3590.3620.1927.80.2950.2780.2870.11700.1730.1670.170 | ||||||
货期：3-5 working days
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