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Human soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA KIT

  • 中文名： 人可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)ELISA KIT
货号: IPD-E04750h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人可溶性肿瘤坏死因子相关凋亡诱导配体(sTRAIL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF10含量。TNFSF10是重要靶点。它属于肿瘤坏死因子超家族成员，在细胞凋亡、免疫调节等方面发挥重要作用。研究发现其可特异性诱导肿瘤细胞凋亡，还涉及免疫监视。目前围绕它的研究机制聚焦于挖掘其信号通路，以开发相关抗癌疗法。试剂盒检测范围为15.6 pg/ml- 1000pg/ml，适用于细胞凋亡机制研究、肿瘤生物学探索及免疫调节相关实验需求，为药物开发中靶向分子效果评估、疾病模型构建等科研场景提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Apo 2 ligand ELISA Kit; APO 2L ELISA Kit; Apo-2 ligand ELISA Kit; Apo-2L ELISA Kit; APO2L ELISA Kit; CD253 ELISA Kit; CD253 antigen ELISA Kit; Chemokine tumor necrosis factor ligand superfamily member 10 ELISA Kit; Protein TRAIL ELISA Kit; TL2 ELISA Kit; TNF Related Apoptosis Inducing Ligand ELISA Kit; TNF related apoptosis inducing ligand TRAIL ELISA Kit; TNF-related apoptosis-inducing ligand ELISA Kit; TNF10_HUMAN ELISA Kit; TNFSF10 ELISA Kit; TRAIL ELISA Kit; Tumor necrosis factor (ligand) family member 10 ELISA Kit; Tumor Necrosis Factor (ligand) Superfamily Member 10 ELISA Kit; Tumor necrosis factor apoptosis inducing ligand splice variant delta ELISA Kit; Tumor necrosis factor ligand superfamily member 10 ELISA Kit
  • 缩写：
    TNFSF10
  • Uniprot No.：
    P50591
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml - 1000pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human sTRAIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    103


    Range %

    99-110


    1:2

    Average %

    96


    Range %

    92-100


    1:4

    Average %

    90


    Range %

    85-94


    1:8

    Average %

    92


    Range %

    88-96


  • 回收率：

    The recovery of human sTRAIL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    88

    84-92


    EDTA plasma (n=4)

    95

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.158

    2.257

    2.208

    2.040


    500

    1.621

    1.720

    1.671

    1.503


    250

    1.103

    1.068

    1.086

    0.918


    125

    0.719

    0.703

    0.711

    0.543


    62.5

    0.462

    0.485

    0.474

    0.306


    31.2

    0.337

    0.319

    0.328

    0.160


    15.6

    0.230

    0.241

    0.236

    0.068


    0

    0.164

    0.172

    0.168




  • 货期：
    3-5 working days

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