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产品描述：山羊肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种具有多种生物活性的细胞因子。它参与免疫调节、炎症反应等生理过程。其研究机制包括与受体结合激活信号通路，调节基因表达。异常的 TNF 表达与多种疾病如类风湿关节炎等相关，也是药物研发的重要靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持科研人员开展山羊炎症模型建立、免疫机制研究、疫苗效力评估等实验，为畜牧兽医研究、动物疾病模型开发及生物制品质量控制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：TNF
Uniprot No.：P13296
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of goat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-97 | ||||
| EDTA plasma (n=4) | 97 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6012.5892.5952.4935002.1542.1332.1442.0422501.6581.6211.6401.5381251.1231.1141.1191.01762.50.7120.6650.6890.58731.20.4130.3990.4060.30415.60.2560.2420.2490.14700.1040.1000.102 | ||||||
货期：3-5 working days
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