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产品描述：犬肿瘤坏死因子α (TNF-α )酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。TNF即肿瘤坏死因子，是一种重要的细胞因子。它在炎症反应、免疫调节等生理病理过程中起关键作用。研究机制上，TNF可通过与受体结合，激活多条信号通路，参与细胞存活、凋亡、增殖等调控，其异常与多种疾病如自身免疫病、肿瘤等相关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品广泛应用于动物模型研究，如炎症性疾病机制探索、免疫抑制剂药效评估、疫苗免疫原性分析等领域，为犬类基础医学研究和生物标志物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：TNF
Uniprot No.：P51742
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-105 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of dog TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-105 | ||||
| EDTA plasma (n=4) | 95 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2112.2352.2232.07851.6721.6841.6781.5332.51.1121.1351.1240.9791.250.6290.6390.6340.4890.6250.3730.3840.3790.2340.3120.2640.2770.2710.1260.1560.1970.2010.1990.05400.1440.1450.145 | ||||||
货期：3-5 working days
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