首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠粘胶蛋白/肌腱蛋白C(TN-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TN-C含量。TN - C是当前靶点。它在细胞外基质中发挥重要作用，与细胞黏附、迁移等过程相关。研究其机制聚焦于它和肿瘤微环境、肿瘤细胞生长及转移的关系，通过揭示TN - C作用机制或为肿瘤等相关疾病的治疗提供新靶点与思路。试剂盒检测范围为2.5 ng/mL-160 ng/mL，科研人员可将其应用于心血管疾病模型、肝脏纤维化研究或创伤修复机制探索，通过检测不同病理状态下TN - C的动态表达，揭示其在细胞黏附、信号转导中的功能，为靶向治疗策略提供基础数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TN-C
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 ng/mL-160 ng/mL
灵敏度：0.625 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 106 | |||||
| Range % | 101-113 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 1602.4172.5022.4602.301 802.1022.1732.1381.979 401.7001.6151.6581.499 201.0851.0491.0670.908 100.6710.6840.6780.519 50.4240.4370.4310.272 2.50.2930.2810.2870.128 00.1620.1560.159 | |||||||
货期：3-5 working days
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