首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛肿瘤坏死因子α（TNF-α）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。TNF即肿瘤坏死因子，是一类能引起肿瘤组织出血坏死的细胞因子。在机体免疫和炎症反应中发挥关键作用。其研究机制主要围绕调节细胞存活、增殖、分化及死亡，还参与炎症信号传导，对其深入研究有助于多种炎症性疾病及肿瘤的治疗。试剂盒检测范围为0.1 ng/mL-20 ng/mL，适用于畜牧科研中炎症相关疾病模型的建立、疫苗免疫效果评估，以及抗炎药物作用机制研究等科研领域，为探索牛类疾病的分子机制提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：TNF
Uniprot No.：Q06599
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.1 ng/mL-20 ng/mL
灵敏度：0.05 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of bovine TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-101 | ||||
| EDTA plasma (n=4) | 95 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1730.1760.175 50.3120.3250.319 1.60.6170.6340.626 0.41.0351.1181.077 0.11.3961.4051.401 02.5132.5462.530 | ||||||
货期：3-5 working days
×
