首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肌腱蛋白(TNC)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNC含量。TNC（Tenascin - C）是一种细胞外基质糖蛋白。在胚胎发育、组织修复等生理过程有重要作用，在肿瘤发生、发展中也扮演关键角色。研究机制主要围绕其与细胞黏附、迁移、增殖及血管生成等过程的关联，通过影响信号通路发挥作用。试剂盒检测范围为9.4 pg/mL-600 pg/mL，可广泛应用于基础研究中TNC在组织再生模型、纤维化病理进程或肿瘤微环境动态变化中的表达规律探索，为解析其分子机制提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnc ELISA Kit; Hxb ELISA Kit; Tenascin ELISA Kit; TN ELISA Kit; Hexabrachion ELISA Kit; Tenascin-C ELISA Kit; TN-C ELISA Kit
缩写：TNC
Uniprot No.：Q80YX1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：9.4 pg/mL-600 pg/mL
灵敏度：2.34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:200 | Average % | 101 | ||||
| Range % | 97-103 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 85-93 | |||||
回收率：
| The recovery of mouse TNC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 94 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average6000.0850.0810.0833000.1790.1710.1751500.2750.2980.287750.5520.5680.56037.50.9630.9370.95018.751.4511.4681.4609.41.7801.7981.78902.5672.5982.583 | ||||||
货期：3-5 working days
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