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产品描述：大鼠心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的cTn-I含量。cTn-I（肌钙蛋白I）是心肌肌肉收缩的调节蛋白，仅在心肌组织中表达，具有高度的心肌特异性和敏感性，是诊断急性心肌梗死（AMI）的首选生化指标。其研究机制主要涉及心肌损伤后cTn-I释放到血液中，通过检测血液中cTn-I水平，可以客观反映心肌损伤情况。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中对心肌损伤机制的研究，支持药物心脏毒性评估、心肌缺血再灌注损伤研究等实验场景，满足实验室对心血管疾病机制探索、病理模型建立等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnni3 ELISA Kit; Ctni ELISA Kit; Tni ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写：cTn-Ⅰ
Uniprot No.：P23693
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.3212.2202.2712.118 10001.3401.4411.3911.238 5000.8140.8350.8250.672 2500.5290.5400.5350.382 1250.3450.3340.3400.187 62.50.2610.2500.2560.103 31.250.1970.1930.1950.042 00.1530.1520.153 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat cTn-I antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled cTn-I antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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