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产品描述：犬心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒 Kit为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的cTn-I含量。cTn - I是心肌肌钙蛋白I，是心肌损伤的特异性标志物。其背景在于在心肌受损时释放入血，可反映心肌损伤情况。研究机制主要聚焦在检测其在血液中的含量变化，用于早期诊断急性心肌梗死等心肌损伤相关疾病。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本产品适用于科研领域，如犬类心肌病机制探索、心脏毒性药物评价或运动医学研究中心肌微损伤监测，为兽医基础研究、实验动物模型构建及比较医学分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写：cTn-
Uniprot No.：Q8MKD5
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-108 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.2452.2502.2482.0717501.7161.7041.7101.5333751.1441.1611.1530.976187.50.7900.7750.7830.606940.4590.4740.4670.290470.3050.2890.2970.12023.50.2150.2110.2130.03600.1760.1770.177 | ||||||
货期：3-5 working days
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