首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胸腺肽β4（Thymosin β4）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TMSB4X含量。TMSB4X 是一种重要靶点。它在细胞增殖、迁移、血管生成等多方面发挥作用。其研究机制主要聚焦于参与细胞内信号转导通路影响细胞行为，还和肿瘤的发生、发展及转移相关，在疾病诊断、治疗及预后评估上有潜在价值。试剂盒检测范围为78 ng/mL-5000 ng/mL，适用于体外科研实验中Thymosin β4的动态浓度监测，如心肌损伤修复模型、皮肤创伤愈合研究或炎症相关疾病的机制探索，也可用于评估特定药物或干预措施对Thymosin β4表达水平的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fx ELISA Kit; Hematopoietic system regulatory peptide ELISA Kit; Prothymosin beta 4 ELISA Kit; PTMB 4 ELISA Kit; PTMB4 ELISA Kit; Seraspenide ELISA Kit; T beta 4 ELISA Kit; T beta-4 ELISA Kit; TB4X ELISA Kit; THYB 4 ELISA Kit; Thyb4 ELISA Kit; Thymosin beta 4 ELISA Kit; Thymosin beta 4 X chromosome ELISA Kit; Thymosin beta 4 X linked ELISA Kit; TMSB 4 ELISA Kit; TMSB4 ELISA Kit; Tmsb4x ELISA Kit; TYB4_HUMAN ELISA Kit
缩写：TMSB4X
Uniprot No.：P62328
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human thymosin β4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-96 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-106 | |||||
回收率：
| The recovery of human thymosin β4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-94 | ||||
| EDTA plasma (n=4) | 102 | 96-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected50002.4112.3122.3622.20625001.8351.7361.7861.63012501.2121.2031.2081.0526250.7030.6940.6990.5433120.3940.4040.3990.2431560.2770.2860.2820.126780.2230.2280.2260.07000.1550.1560.156 | ||||||
货期：3-5 working days
×
