首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人toll样受体2(TLR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TLR2含量。TLR2即Toll样受体2，是天然免疫中的关键模式识别受体，广泛分布于免疫细胞表面，能够识别病原体相关分子模式。在研究机制上，其与配体结合后触发信号级联反应，激活免疫细胞，调节炎症因子释放，在抗感染、肿瘤免疫等方面研究前景大。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于基础医学研究、药物开发评估及疾病机理探索等科研场景，例如通过检测感染模型动物血清或炎症组织匀浆中的TLR2动态变化，解析免疫应答机制；或评估候选化合物对TLR2信号通路的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD282 ELISA Kit; CD282 antigen ELISA Kit; TIL 4 ELISA Kit; TIL4 ELISA Kit; TLR 2 ELISA Kit; TLR2 ELISA Kit; TLR2_HUMAN ELISA Kit; Toll like receptor 2 ELISA Kit; Toll like receptor 2 precursor ELISA Kit; Toll-like receptor 2 ELISA Kit; Toll/interleukin 1 receptor like 4 ELISA Kit; Toll/interleukin 1 receptor like protein 4 ELISA Kit; Toll/interleukin receptor like protein 4 ELISA Kit; Toll/interleukin-1 receptor-like protein 4 ELISA Kit
缩写：TLR2
Uniprot No.：O60603
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 89-97 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6322.6212.6272.504 102.0682.0562.0621.939 51.1511.1321.1421.019 2.50.6730.6390.6560.533 1.250.4270.4240.4260.303 0.6250.2550.2450.2500.127 0.3120.1890.1770.1830.060 00.1250.1210.123 | ||||||
货期：3-5 working days
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