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产品描述：大鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMP2含量。TIMP2 即金属蛋白酶组织抑制因子 2，在细胞外基质代谢、细胞增殖等生理过程起重要作用。研究发现其可抑制基质金属蛋白酶活性，在肿瘤等疾病中表达异常。它参与肿瘤侵袭、转移等机制，有望成为相关疾病诊断、治疗的潜在靶点。试剂盒检测范围为1.25 ng/mL-80 ng/mL，科研人员可将其应用于纤维化疾病模型研究、炎症反应机制探索或肿瘤微环境分析等领域，通过检测TIMP - 2水平变化揭示其在基质代谢调控中的作用，为心血管疾病、肝纤维化等病理机制研究提供实验依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
缩写：TIMP2
Uniprot No.：P30121
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 88-95 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 94-103 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of rat TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-94 | |||||
| EDTA plasma (n=4) | 96 | 94-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected802.1842.1892.1872.009401.4861.4561.4711.293200.9250.9940.9600.782100.5450.5880.5670.38950.4310.4430.4370.2592.50.3080.2920.3000.1221.250.2510.2330.2420.06400.1790.1770.178 | |||||||
货期：3-5 working days
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