首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶抑制因子3(TIMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMP3含量。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，在肿瘤转移、心血管疾病及组织重塑等研究中具有重要价值；适用于基础科研中探索疾病模型（如纤维化、肿瘤微环境）或药物干预下TIMP - 3的动态表达，为研究其与组织稳态调控、病理进程的关联提供可靠工具；满足实验室对小鼠来源样本的高通量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Timp3 ELISA Kit; Sun ELISA Kit; Timp-3 ELISA Kit; Metalloproteinase inhibitor 3 ELISA Kit; Tissue inhibitor of metalloproteinases 3 ELISA Kit; TIMP-3 ELISA Kit
缩写：TIMP3
Uniprot No.：P39876
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 95-104 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 88-98 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 90-102 | ||||||
回收率：
| The recovery of mouse TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 87-100 | |||||
| EDTA plasma (n=4) | 101 | 95-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.4942.4212.4582.28815001.8931.8561.8751.7057501.1651.1321.1490.9793750.6500.6430.6470.477187.50.4130.3900.4020.23293.750.3010.2940.2980.12846.880.2290.2250.2270.05700.1690.1710.170 | |||||||
货期：3-5 working days
×
