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产品描述：人可溶性胸苷激酶1(TK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TK1含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，肿瘤发生发展机制研究、细胞周期调控、抗肿瘤药物评估、肿瘤生物学研究、细胞增殖相关信号通路探索、药理学实验中药物干预效果的定量评估、体外细胞模型、动物实验组织样本或生物体液中的TK1动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cytosolic ELISA Kit; KITH_HUMAN ELISA Kit; Thymidine kinase 1 ELISA Kit; Thymidine kinase 1 soluble ELISA Kit; Thymidine kinase 1 soluble isoform ELISA Kit; Thymidine kinase ELISA Kit; Thymidine kinase cytosolic ELISA Kit; TK 1 ELISA Kit; TK 2 ELISA Kit; TK1 ELISA Kit; Tk1a ELISA Kit; Tk1b ELISA Kit; TK2 ELISA Kit
缩写：TK1
Uniprot No.：P04183
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 90-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human TK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 104 | 102-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5972.7452.6712.52020002.1552.0882.1221.97110001.4781.5681.5231.3725000.8360.8980.8670.7162500.6410.6110.6260.4751250.4320.4220.4270.27662.50.2220.2180.2200.06900.1470.1540.151 | ||||||
货期：3-5 working days
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