首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人踝蛋白(TLN1)酶联免疫试剂盒为 双抗夹心法 ELISA试剂盒，定量 检测血清、血浆、组织匀浆、细胞裂解物样本中的TLN1含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于细胞生物学实验中的粘着斑动态研究、肿瘤微环境分析、心血管疾病机制探索等科研场景，为体外研究TLN1在细胞力学响应、病理生理过程中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ILWEQ ELISA Kit; Talin 1 ELISA Kit; Talin ELISA Kit; Talin-1 ELISA Kit; TLN 1 ELISA Kit; TLN ELISA Kit; Tln1 ELISA Kit; TLN1_HUMAN ELISA Kit
缩写：TLN1
Uniprot No.：Q9Y490
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-101 | |||||
回收率：
| The recovery of human TLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.4282.4682.4482.2847501.6511.6981.6751.5113751.1041.1001.1020.938187.50.6620.6570.6600.496940.4770.4980.4880.324470.3550.3620.3590.19523.50.2610.2710.2660.10200.1650.1630.164 | ||||||
货期：3-5 working days
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