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产品描述：小鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TIMP2含量。TIMP2 是组织金属蛋白酶抑制因子 2，在体内能抑制金属蛋白酶活性，维持细胞外基质稳态。其研究机制主要聚焦于它在肿瘤发生、发展中作用，如抑制肿瘤细胞侵袭、转移，还参与血管生成调控，有望成为癌症等疾病治疗新靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外细胞实验、动物模型研究及组织样本分析，为探究TIMP-2在炎症反应、肿瘤微环境调控或器官纤维化等领域的分子机制提供可靠工具，适用于分子生物学、病理机制研究等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
缩写：TIMP2
Uniprot No.：P25785
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 106 | ||||
| Range % | 101-113 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:80 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:160 | Average % | 98 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 88 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 80002.2182.3512.2852.110 40001.6741.6071.6411.466 20001.2411.1941.2181.043 10000.8020.8530.8280.653 5000.5760.6120.5940.419 2500.3490.3400.3450.170 1250.2610.2670.2640.089 00.1770.1720.175 | ||||||
货期：3-5 working days
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