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产品描述：猪基质金属蛋白酶抑制因子1(TIMP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMP1含量。TIMP1即金属蛋白酶组织抑制因子1，它在维持细胞外基质稳态中起关键作用。其表达异常与肿瘤发生、转移及预后等密切相关。研究机制上，TIMP1可抑制基质金属蛋白酶活性，还能通过多种信号通路影响细胞增殖、凋亡等生物学行为。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于心血管疾病、肝脏纤维化、肿瘤微环境等领域的科研探索，为研究组织再生机制、炎症相关信号通路或疾病模型中的基质重塑提供定量工具，满足体外实验中对TIMP - 1蛋白表达水平的动态监测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
缩写：TIMP1
Uniprot No.：P35624
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-107 | |||||
回收率：
| The recovery of pig TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 85-99 | ||||
| EDTA plasma (n=4) | 97 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.4222.3952.4092.2362501.4661.5061.4861.3131250.9861.0070.9970.82462.50.6110.5890.6000.42731.20.4250.4540.4400.26715.60.3420.3620.3520.1797.80.2420.2500.2460.07300.1750.1700.173 | ||||||
货期：3-5 working days
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