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产品描述：人T细胞免疫球蛋白及粘蛋白域包含蛋白4(TIMD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIMD4含量。TIMD4，又称TIM-4，是一种膜蛋白，作为磷脂酰丝氨酸受体，在免疫应答中发挥作用，如凋亡细胞的吞噬和T细胞调节。TIMD4通过双模式控制T细胞活化，并促进对凋亡细胞或外源颗粒的吞噬，对T细胞活化和免疫反应至关重要。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域中对免疫细胞功能调控机制、感染性疾病免疫应答、肿瘤免疫逃逸等方向的研究，为探索TIMD4在病理生理过程中的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SMUCKLER ELISA Kit; Spleen; mucin-containing; knockout of lymphotoxin protein ELISA Kit; T cell immunoglobulin and mucin domain containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing molecule ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domains-containing protein 4 ELISA Kit; T-cell membrane protein 4 ELISA Kit; TIM-4 ELISA Kit; Tim4 ELISA Kit; TIMD 4 ELISA Kit; TIMD-4 ELISA Kit; Timd4 ELISA Kit; TIMD4_HUMAN ELISA Kit
缩写：TIMD4
Uniprot No.：Q96H15
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TIMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-91 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human TIMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 82-94 | ||||
| EDTA plasma (n=4) | 100 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5172.4762.4972.35151.5831.5431.5631.4172.50.9511.0050.9780.8321.250.6020.6370.6200.4740.6250.4810.4660.4740.3280.3120.2910.3040.2980.1520.1560.2120.2170.2150.06900.1480.1440.146 | ||||||
货期：3-5 working days
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