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产品描述：人血小板反应素1型结构域蛋白4(THSD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的THSD4含量。THSD4是一种预测具有水解酶活性和细胞外基质结构成分的蛋白，可能在弹性纤维组件中起作用，位于含胶原蛋白的细胞外基质和细胞外外泌体中。其研究机制涉及遗传性血小板病，如巨血小板综合征和血小板无力症，以及其在止血、炎症和免疫反应中的作用。试剂盒检测范围为12.5 pg/mL-800 pg/mL，该产品适用于基础研究中对THSD4表达水平的定量分析，如探究其在心血管疾病、纤维化病变或肿瘤进展中的分子机制，亦可用于体外培养细胞模型中THSD4分泌水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：THSD4 ELISA Kit; UNQ9334/PRO34005 ELISA Kit; Thrombospondin type-1 domain-containing protein 4 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 ELISA Kit; ADAMTS-like protein 6 ELISA Kit; ADAMTSL-6 ELISA Kit
缩写：THSD4
Uniprot No.：Q6ZMP0
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human THSD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:40 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:80 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:160 | Average % | 102 | ||||
| Range % | 97-107 | |||||
回收率：
| The recovery of human THSD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 95-110 | ||||
| EDTA plasma (n=4) | 93 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 8002.6752.8702.7732.674 4002.3242.3452.3352.236 2001.6181.6551.6371.538 1000.8640.9010.8830.784 500.5020.4960.4990.400 250.3240.3450.3350.236 12.50.1980.2000.1990.100 00.1010.0960.099 | ||||||
货期：3-5 working days
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