首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶1(Tie-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TIE1含量。TIE1是一个靶点。它在血管生成等生理过程中发挥作用。研究发现其与血管内皮细胞的存活、增殖等机制密切相关。深入研究TIE1有助于了解血管相关疾病发病机制，为开发针对该靶点治疗血管疾病的药物提供方向。试剂盒检测范围为0.94 ng/mL-60 ng/mL，适用于基础科研中探索血管生成机制、评估心血管疾病模型血管内皮功能变化，或分析肿瘤微环境中的血管重塑等相关研究，为血管生物学、肿瘤学及病理生理学领域的体外研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：JKT 14 ELISA Kit; JTK14 ELISA Kit; TIE ELISA Kit; Tie1 ELISA Kit; TIE1_HUMAN ELISA Kit; Tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 ELISA Kit; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains ELISA Kit; Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 ELISA Kit; Tyrosine protein kinase receptor Tie 1 ELISA Kit; Tyrosine-protein kinase receptor Tie-1 ELISA Kit
缩写：TIE1
Uniprot No.：P35590
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.94 ng/mL-60 ng/mL
灵敏度：0.23 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Tie-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:5 | Average % | 87 | |
| Range % | 83-92 | ||
| 1:10 | Average % | 106 | |
| Range % | 101-110 | ||
| 1:20 | Average % | 99 | |
| Range % | 95-103 | ||
| 1:40 | Average % | 98 | |
| Range % | 94-103 |
回收率：
| The recovery of human Tie-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 91 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 602.5312.5012.5162.402 302.0182.1212.0701.956 151.5051.5681.5371.423 7.51.0401.0681.0540.940 3.750.6570.6870.6720.558 1.880.4210.4320.4270.313 0.940.2710.2900.2810.167 00.1120.1150.114 | ||||||
货期：3-5 working days
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