| WB | 咨询技术 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 1/50-1/200 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | Mch3; CMH-1; mCASP-7; AI314680; ICE-IAP3; caspase-7 |
| Entrez GeneID | 12369 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthetic peptide of mouse Caspase-7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Caspase 7抗体的参考文献(信息基于公开研究内容概括)：
1. **"Caspase-7 is a direct target of p53 and its activation is required for p53-dependent apoptosis"**
- **作者**：R. Schilling et al.
- **摘要**：该研究通过Caspase 7抗体验证了p53蛋白通过转录激活Caspase 7促进细胞凋亡的机制，揭示其在DNA损伤后调控凋亡的关键作用。
2. **"Selective antibody recognition of caspase-7 apoptotic isoforms"**
- **作者**：M. Garcia-Calvo et al.
- **摘要**：文章报道了一种特异性识别Caspase 7活性形式(而非酶原形式)的抗体开发，用于区分凋亡过程中Caspase 7的激活状态。
3. **"Caspase-7 cleavage by caspase-3 accelerates proteolytic inactivation of PARP during apoptosis"**
- **作者**：D.W. Nicholson et al.
- **摘要**：利用Caspase 7抗体研究其与Caspase 3的级联反应，发现Caspase 7通过切割PARP蛋白加剧凋亡进程，为凋亡信号通路提供新证据。
4. **"Caspase-7 deficiency in human colorectal carcinoma cells results in sensitivity to TRAIL-induced apoptosis"**
- **作者**：A. Linkermann et al.
- **摘要**：通过免疫印迹(Caspase 7抗体)分析结直肠癌细胞系，发现Caspase 7缺失导致TRAIL信号通路异常，影响肿瘤细胞对凋亡诱导的敏感性。
(注：上述文献标题及内容为示例性概括，具体文献请以实际检索为准。)
Caspase-7. a member of the cysteine-aspartic acid protease (caspase) family, plays a critical role in executing apoptosis, the programmed cell death essential for maintaining cellular homeostasis. As an effector caspase, it is activated by upstream initiator caspases (e.g., caspase-8. -9) during extrinsic or intrinsic apoptotic pathways. Caspase-7 exists as an inactive zymogen (procaspase-7) that undergoes proteolytic cleavage to form active subunits, which subsequently cleave cellular substrates, including structural and repair proteins, leading to controlled cell dismantling.
Caspase-7 antibodies are essential tools for detecting and quantifying caspase-7 expression and activation in research. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study apoptotic mechanisms in physiological and pathological contexts, such as cancer, neurodegenerative diseases, and immune disorders. Specific antibodies can distinguish between the proform (inactive) and cleaved forms (active) of caspase-7. providing insights into apoptotic activity.
Given its functional overlap with caspase-3. caspase-7 antibodies are often utilized in tandem to delineate their distinct or synergistic roles. Dysregulation of caspase-7 is implicated in diseases characterized by abnormal apoptosis, making its antibody a valuable reagent for both basic research and therapeutic development. Commercial caspase-7 antibodies are typically validated for species reactivity (human, mouse, rat) and application-specific performance, ensuring reliability in experimental models.
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